Enrichment of fetal and maternal long cell‐free DNA fragments from maternal plasma following DNA repair
نویسندگان
چکیده
منابع مشابه
Size distributions of maternal and fetal DNA in maternal plasma.
BACKGROUND The discovery of fetal DNA in maternal plasma has opened up an approach for noninvasive prenatal diagnosis. Despite the rapid expansion in clinical applications, the molecular characteristics of plasma DNA in pregnant women remain unclear. METHODS We investigated the size distribution of plasma DNA in 34 nonpregnant women and 31 pregnant women, using a panel of quantitative PCR ass...
متن کاملFetal DNA clearance from maternal plasma is impaired in preeclampsia.
BACKGROUND Increased fetal DNA in maternal plasma/serum has been reported in pregnancies complicated by preeclampsia. We hypothesized that impaired clearance of fetal DNA might contribute, at least in part, to the above-mentioned phenomenon. METHODS We studied 7 preeclamptic and 10 control pregnant women. All had male fetuses. Serial blood samples were obtained from before delivery to 6 h pos...
متن کاملPresence of fetal DNA in maternal plasma and serum.
BACKGROUND The potential use of plasma and serum for molecular diagnosis has generated interest. Tumour DNA has been found in 'the plasma and serum of cancer patients, and molecular analysis has been done on this material. We investigated the equivalent condition in pregnancy-that is, whether fetal DNA is present in maternal plasma and serum. METHODS We used a rapid-boiling method to extract ...
متن کاملLack of dramatic enrichment of fetal DNA in maternal plasma by formaldehyde treatment.
variations in detection sensitivity and quantification of fetal DNA concentrations were noted among laboratories very familiar with real-time PCR technology (21). Further studies will be needed to clarify this issue in addition to the continued exploration of other strategies for the investigation of complex fetal genetic traits by analysis of maternal plasma. These strategies may include the e...
متن کاملFetal DNA in maternal plasma: biology and diagnostic applications.
BACKGROUND Molecular analysis of plasma DNA during human pregnancy has led to the discovery that maternal plasma contains both fetal and maternal DNA. This valuable source of fetal DNA opens up new possibilities for noninvasive prenatal diagnosis. APPROACH Published data from the last 3 years demonstrating the feasibility and utility of analyzing fetal DNA in maternal plasma are reviewed. C...
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ژورنال
عنوان ژورنال: Prenatal Diagnosis
سال: 2019
ISSN: 0197-3851,1097-0223
DOI: 10.1002/pd.5406